For this version of the HomeScope we implement the most basic illumination set up possible. We use a single white LED which is placed under the sample and where a single potentiometer is used to regulate light intensity directly from hardware. The source of power connected to the potentiometer comes from the Raspberry Pi 3B’s only PWM capable pin which we use to control voltage. The average voltage from this pin ranges from 0 to 3.3 Volts. Controlling this pin from software allows HomeScope to automatize light conditions on the sample. Note we chose focal lens with a coating filtering light. Thus we can protect the RaspiCAM from UV and blue LED damage. Once the camera is protected by the optical system, the HomeScope is ready for fluorescent microscopy.

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