We used OpenBeam for beams and brackets. The main frame of the microscope can be decomposed into three different horizontal components that are stack together in a vertical fashion by four 300mm beams holding a Top frame and a Bottom frame. These two frames generate a Z axis where displacement of the Stage up and down the axis. This is controlled by a linear translation mechanism which moves along a couple of smooth rods, acting as linear rails. The Stage is pushed/pulled by a lead screw and a flexible shaft coupler and powered by a stepper motor.
To hold all the beams together we use Openbeam brackets on each of the three horizontal components: Top frame, Bottom frame, and Stage. The Stage, controls XY movement via two micro servos and a 3D printed gear mechanism from the PlotterBot.
Beside beams and brackets from OpenBeam, we need lots of nuts & bolts as well as some custom made components for mechanical support which are not provided by OpenBeam.
Other mechanical components
Linear platform bearings: 2x (Stage)
Shaft coupler: 1x
Adaptor for lead screw to nema bracket on stage (custom made)
Acrylic for (laser cutted) stage board (custom made)
Smooth rod: 2xLead screw + nut: 1x
Nema17 200 steps: 1x (Z axis) Micro Servos 9g: 2x (X,Y axes)
3D printed pieces
PlotterBot pieces: 6x (use the OLD version of the Plotterbot with 2 instead of 3 servos)
Cambridge OpenLabToolsoptical clamp: 1x
Cambridge OpenLabToolsRaspiCAM holder: 1x
Optical tube (instead of the Edmund Optics metal tubes)
Edmund C-mount-DIN optical system (tubes in case of not printing them)
Comar Optics Focal lens
Objectives from CNScopes (4x,10x)
Arduino UNO: 2x Easy Driver: 1x Raspberry Pi 3B: 1x
RaspiCAM: 1x Logical convertor: 3x Joystick: 1x
i2c 16×2 LCD: 1x Breadboards: 3x + 3x Cobbler Pi PIN cable: 1x
Push button: 2x resistors (for LEDs and for pull down the push buttons)
White LED: 1xPotentiometer: 1x